Fig. 4. Expression of RYR subtypes in duodenum myocytes. (A) cDNA fragments of RYR subtypes were amplified from duodenum myocytes. cDNA fragments were separated on a 2% agarose gel, visualized by staining with ethidium bromide and shown as a contrasted image. Molecular size standard is 174-HinfI scale. (B) Compiled data of total fluorescence (white bars) and non-specific fluorescence (hatched bars) observed in cells with the anti-RYR1 (1:1000), anti-RYR2 (1:500) and anti-RYR3 (1:1000) antibodies. Data are means ± s.e.m. with the number of tested cells indicated in parentheses (stars indicate P<0.05). AU, arbitrary units. (C) Typical confocal sections showing immunostaining obtained with anti-RYR1 (1:1000), anti-RYR2 (1:500) and anti-RYR3 (1:1000) antibodies. Images were contrasted with Confocal Assistant 4.2 software. The same correction factors were applied on all the images. (D) Compiled data of specific fluorescence observed in non-injected cells (white bars) and injected cells (hatched bars). Three days after injection of antisense oligonucleotides, myocytes were stained with anti-RYR1 (left bars), anti-RYR2 (middle bars) and anti-RYR3 (right bars) antibodies. Data are means ± s.e.m. with the number of tested cells indicated in parentheses (stars indicate P<0.05). AU, arbitrary units.