Fig. 5. Effects of antisense oligonucleotides directed against the RYR subtypes on Ca²⁺ oscillations induced by caffeine, ACh and cADPR. Compiled data showing the amplitude of Ca²⁺ responses evoked by 10 mM caffeine (A), 1 µM ACh (B), 100 nM cADPR (C) in non-injected cells (white bars) and cells injected with 10 µM asRYR antisense oligonucleotides (black bars). Results in A and B were obtained in intact cells and results in C were obtained in permeabilized cells. Data are means ± s.e.m. with the number of tested cells indicated in parentheses. Myocytes were loaded with 2 µM Fluo4-AM and used 3 days after oligonucleotide injection (stars indicate P<0.05).