(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Western blot analysis of IP3R1 in PM and MA muscle fiber cultures. (A) Whole cell protein extracts (200 µg for IP3R1 and 10 µg for {alpha}-actin) from innervated (+SC) and non-innervated (–SC) PM and medial adductor MA muscle fibers were electrophoresed and immunoblotted. IP3R1 and {alpha}-actin were detected with a rabbit polyclonal antibody and mouse polyclonal antibody, respectively, followed by HRP-conjugated secondary antibodies and chemiluminescent substrates. (B) The abundance of IP3R1 in innervated (+SC) and non-innervated (–SC) PM and MA muscle fibers was quantified by using {alpha}-actin as a loading control for normalization Innervation significantly (P<0.05) increased IP3R1 abundance in both PM and MA muscle fibers, and IP3R1 abundance was significantly (P<0.05) increased in innervated and non-innervated PM muscle fibers relative to that in MA muscle fibers. Bars represent mean±s.e.m., n=4.