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Fig. 2. Absence of S1 fibers in vimentin-deficient cells. Vimentin-deficient tTA-1 mouse fibroblasts (–/–), and control vimentin (+/+) MFT-6 fibroblasts were double-stained with anti-S1 protein antibody McAb 351 (left) and an anti-vimentin antibody (middle). Images were obtained by confocal microscopy. Merged images are shown on the right.