Fig. 4. Immunoprecipitation of vimentin with McAb 351. ARL cell extract was immunoprecipitated with control Tris-buffered saline (lane 1), control antibody I-65 against toxoplasma membrane (lane 2), control 3F7 against an RNA-binding protein RBP-MS (lane 3) or McAb 351 against S1 proteins C2 and D2 (lane 4) and the precipitated proteins were immunoblotted for vimentin. Lane 5, reference total proteins from ARL cells. Positions of molecular weight protein markers (M) are indicated on the right-hand side of the blot.