Fig. 8. Appearance of S1 proteins in the cytoplasm of migratory fibroblasts. (A-H) Fibroblasts in the healing tissue of gastric ulcer (left panels) and those in the sham-operated intact tissue of the stomach (right panels) were stained with McAb 351 (A-D) and with an anti-vimentin antibody (E-H). Nuclei (arrows) were counterstained with Methyl Green. Spindle-shaped fibroblasts were cut along the short (A,B,E,F) and long axes (C,D,G,H). In addition to the nucleus, the cytoplasm was stained with McAb 351 in ulcerated healing tissue (A,C compare with B,D). Vimentin was present in the cytoplasm of both ulcerated (E,G) and intact (F,H) tissues, with more vimentin in the former. (I) Vimentin mRNA levels during gastric ulcer healing. Total RNA from gastric tissues on day 5 after ulcer formation was analyzed by Northern blotting for vimentin and GAPDH. One representative autoradiogram of six determinations is shown. Bar, 12.5 µm.