Fig. 4. Subcellular distribution of GFP fusion proteins containing either the N-terminal domain A or derived subdomains, or full-length or n-truncated SCG10 cells. MDCK cells were transfected with (A) A-GFP, a_nm-GFP, a_m-GFP, a_mc-GFP and (B) SCG10-GFP or n-SCG10-GFP constructs, fixed after 24 hours and GFP fluorescence was examined by fluorescence confocal microscopy (A) (images are projections from confocal stacks) or (B) conventional fluorescence microscopy. The subcellular distribution of the proteins is very similar to that observed in HeLa cells: A-GFP (A), a_nm-GFP (a_nm) and the full-length SCG10-GFP are mainly restricted to the Golgi complex and punctate structures (in the case of SCG10-GFP, the Golgi is disseminated owing to the microtubule-destabilizing activity of the transfected protein), whereas a_m-GFP (a_m), a_mc-GFP (a_mc) and n-SCG10-GFP are also present at the plasma membrane. Bar, 10 µm.