Fig. 4. T735E.TACE-eGFP colocalises with components of the cells protein secretory pathway. HeLa cells were transfected with 3 µg pT735E.TACE-eGFP and incubated at 37°C for 24 hours. Cells were fixed in methanol, followed by immunostaining with antibodies specific for the ER (calregulin), COPII vesicles (Sec-23) and trans-Golgi network (TGN-46) and visualised using laser-scanning confocal microscopy. T735E.TACE-eGFP and the subcellular markers are highlighted in green and red (respectively) whereas the nuclei are shown in blue. Positive colocalisation is highlighted with white arrowheads. Bar, 10 µm.