Fig. 10. When cytosol is present, clathrin and AP2 rebind to the identical clathrin-coated pit. Permeabilized GFP-clathrin-cells (a-h) were uncoated and fixed, and simultaneously stained with anti-clathrin and anti-AP2 antibodies. Clathrin (b,f) was stained with CHC5.9 antibody and AP2 (c,g) was stained with AP.6 antibody. For double-staining, CHC5.9 and AP.6 were visualized with cy5-conjugated anti-mouse IgM antibody and rhodamine-conjugated anti-mouse IgG1, respectively.