Fig. 2. Uncoating of clathrin-coated pits requires Hsc70, auxilin and ATP. Factors known to affect the uncoating of CVs were incubated with the permeabilized cells for 5 minutes, in an attempt to uncoat permeabilized cells. Conditions were buffer A plus ATP, 1 mM; Hsc70, 2 µM; auxilin, 0.2 µM as indicated. Bars, 10 µm.