JCS -- Yim et al. 118 (11): 2405 Figure IG9

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Fig. 9. AP2 and epsin rebind to clathrin-coated pits that were uncoated by cytosol. Cells were treated with bovine-brain cytosol (10 mg/ml) and ATP (1 mM) for 5 minutes. (A) GFP-AP2 (a-c) and GFP-epsin (d-f) dissociate from puncta in the presence of cytosol and ATP. (B) Cytosol-induced release of AP2 ( ${diamond}$ , ${diamondsuit}$ ) and epsin ( ${triangleup}$ , ${blacktriangleup}$ ) was measured at 20-second intervals in the presence ( ${diamond}$ , ${triangleup}$ ) and absence ( ${diamondsuit}$ , ${blacktriangleup}$ ) of YDJ1. The dashed line represents the release of clathrin, obtained under conditions identical to the ones described above. (C) AP2 and epsin release by cytosol are accompanied by their rebinding. Cells were treated with bovine-brain cytosol and 1 mM ATP for 5 minutes, then fixed and immunostained. Permeabilized cells with GFP-AP2 (a,b,e,f) and GFP-epsin (c,d,g,h) are shown before (a-d) and after (e-h) treatment with cytosol. GFP-fluorescence (a,c,e,g) and immunostaining (b,d,f,h) are shown.