Fig. 3. Palmitoylation enhances transport of PLP and DM20 to MLMs. Primary oligodendrocytes were infected with recombinant SFV to express the palmitoylation-deficient form of DM20, DM20(C5,6,9,108S)-myc (A), and DM20-myc (B). Cells were stained for PLP (O10) and MBP to visualize MLM (arrows). Staining with anti-myc antibody revealed preferential targeting of DM20-myc to MLMs compared with DM20(C5,6,9,108S)-myc. Primary oligodendrocytes were infected with SFV-DM20-myc or SFV-DM20(C5,6,9,108S)-myc and light-membrane fractions were isolated according to the myelin-isolation protocol to enrich for MLMs. The amount of virus-derived DM20-myc and DM20(C5,6,9,108S)-myc in the myelin preparations was determined by immunoblotting with an anti-myc antibody (C). (D) DM20-myc and DM20(C5,6,9,108S)-myc with palmitoylation sites are shown in red. Bar, 20 µm.