Fig. 5. Transport of acylated GFP fusion proteins to MLMs. Primary oligodendrocytes were transiently transfected with plasmids coding for various fluorescent fusion proteins containing different acylation sites. We used a EGFP fusion protein with the C-terminal 20 amino acids of H-Ras (EGFP-tH), which contain a combined farnesylation/palmitoylation signal, and an EYFP fusion protein with the N-terminal 20 amino acids of neuromodulin (tN-EYFP), which contain a consensus sequence for dual palmitoylation. To visualize MLMs, cells were stained for PLP (O10) and MBP. Both EGFP-tH (A) and tN-EYFP (B) were found in MLMs. By contrast, an LDL-receptor construct in which the cytoplasmic domain of the LDL receptor had been replaced by the cytoplasmic domain of CD46 to remove its endocytic sorting determinant and the extracellular LDL receptor domain had been replaced by EGFP was not localized to MLMs (C,D). (E) The constructs used. Bar, 10 µm.