Fig. 6. Palmitoylation-deficient mutants of PLP/DM20 are less efficiently targeted to myelin in myelinating co-cultures of oligodendrocytes and neurons. Myelinating co-cultures of oligodendrocytes and neurons were infected with recombinant SFV to produce 1-13PLP(C5,6,9S)-EYFP (A), 1-13PLP-EYFP (B), DM20(C5,6,9,108S)-myc (C) and DM20-myc (D). The myelin sheaths and axons were identified by labelling with anti-MBP antibody and with an antibody specific for neuronal tubulin or neurofilament, respectively. Bars, 20 µm.