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Fig. 2. Effective detection of low-abundance mRNA using FISH-TSA. Images show representative chicken embryo fibroblasts processed using DIG-labelled sense (A) or antisense (B) RNA probe for chicken Arp3 mRNA. White dots in A mark the cell border. Blue indicates DAPI staining of nucleus, red indicates Arp3 mRNA in the cell and the arrow points to the mRNA localized to protrusions. Bar, 10 µm.