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Fig. 5. Arp2/3-complex and ß-actin mRNAs are localized to protrusions in the cell but do not colocalize. Double detection of two types of mRNAs in the same cell was performed by using both DIG-labelled and fluorescein-labelled probe for FISH. TSA was performed sequentially. (A) Chicken Arp3 mRNA. (B) Chicken ß-actin mRNA. (C) Merge of A and B. (D) Chicken Arp3 mRNA. (E) Chicken Arp2 mRNA. (F) Merge of D and E. Bar, 10 µm.