Fig. 6. Overexpression of DmCdc6 in S2 cells. Two independent transformed S2 cell lines (S2 Cdc6-1 and S2 Cdc6-2) were analysed for expression of DmCdc6 either with or without induction by copper. The protein levels were visualised by western blotting with antibody against either the DmCdc6 protein itself or the His tag present in the overexpressed protein. In each case total cell extract from 500,000 cells was loaded. The western was also developed with anti tubulin antibody as a loading control.