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Fig. 2. Expression of wild-type and mutant forms of FcRn {alpha} chain in IMCD cells. Stable cell lines were selected after transfection of IMCD cells with cDNA encoding the {alpha} chain of wild-type or mutant FcRn. Extracts of these cells were analyzed on western blots using polyclonal rabbit antiserum against rat FcRn {alpha} chain. The two forms of FcRn detected differ in their glycosylation (see Results).