Fig. 3. Analysis of the specificity of interaction between centaurin-₁ and KIF13B by yeast two-hybrid assay. Centaurin-₁ or cytohesin 2 fused with the DNA binding domain of LexA (Bait) were co-transformed with KIF13B/IPCEF-1/IPCEF-2 fused to the activation domain of Gal4 (Prey) into yeast (L40) and then we analysed the ability of the transformants to grow on solid synthetic drop-out medium lacking histidine, and to produce ß-galactosidase as assessed by conversion of X-gal (5-bromo-4-chloro-3-indolyl-ß-D-galactoside) into a blue-coloured product. The panels left to right show three independent clones of each transformant grown in the presence (+H) and absence (–H) of histidine, and the filter ß-galactosidase assay (ß-gal). This analysis was performed three times with three different yeast transformants, with identical results.