JCS -- Venkateswarlu et al. 118 (11): 2471 Figure IG4

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Fig. 4. Centaurin- ${alpha}$ ₁ directly binds KIF13B in vitro. (A) ³⁵S-radiolabelled KIF13B ${Delta}$ C (aa 1-991) was expressed using an in vitro translation system and incubated with glutathione beads immobilized with either GST or GST-centaurin- ${alpha}$ ₁. The beads were washed and bound protein was resolved by SDS-PAGE and detected by fluorography. (B,C) Purified recombinant centaurin- ${alpha}$ ₁ was incubated with beads linked to GST or GST-KIF13B ${Delta}$ . After washing the beads, protein bound to the beads was resolved by SDS-PAGE and analysed by Coomassie Blue staining (B) or immunostaining using an anti-centaurin- ${alpha}$ ₁ antibody (C). The experiment was repeated two further times with identical results.