Fig. 8. Interaction with KIF13B is not conserved among ARF GAPs. COS cells were transiently transfected with the indicated expression vectors. After 2 days, cells were lysed and incubated with GST-tagged KIF13B coupled to glutathione beads. After washing the beads, the bound proteins were analysed by immunoblotting using an anti-GFP antibody (right-hand panel). 5% of the input (cell lysates) was also immunoblotted using an anti-GFP antibody to ensure that the indicated GFP-tagged ARF GAPs were expressed (left-hand panel). Positions of molecular mass standards (kDa) are shown. This analysis was performed three times with identical results.