Fig. 2. Oocyte extracts are depleted in PP2A but retain PP1 after incubation with microcystin beads. Prophase oocytes (Pro) or Metaphase II-arrested oocytes (MII) were homogenized in the presence (+OA) or in the absence of 10^–6 M okadaic acid. These extracts were incubated once (1), twice (2) or three times (3) for 1 hour at 4°C, either with microcystin-agarose beads (A) or with control beads (B). The supernatants (left panels) and the pellets (right panels) were analysed by western blotting with the anti-Cyclin B2 antibody, the anti-Aurora-A antibody, the anti-PP1 antibody and the anti-PP2A antibodies as indicated.