Fig. 6. TEM analysis of PfEMP1 distribution on the surface of K⁺ and K^– IEs. (A) Gold particle clusters decorate the exterior surface of K⁺ IE plasma membrane immediately adjacent to electron dense knobby protrusions (see arrows). Gold particle clusters are observed in the K^– clone on the exterior face of IEs (see arrows), although no electron dense knobby protrusions are present. Bars, 200 nM. (B) The distribution of the numbers of gold particle clusters per IE was determined for both K⁺ and K^– clones. The 25th-75th percentile of the distribution (box), the mean (line within box) and overall range of this distribution is indicated. (C) The distribution of gold particles within each cluster was determined for K⁺ and K^– IEs. The 25th-75th percentile of the distribution (box), the mean (line within box) and overall range of this distribution is indicated.