(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. Competitive leptin-SEAP binding assay of leptin mutants. We tested the binding of leptin mutants using a competitive leptin-SEAP assay as described in the Materials and Methods section. Wild-type leptin and leptin mutants that bind to CRH2 lead to a decreased binding of leptin-SEAP, measured as decreased alkaline phosphatase activity. The L86 mutants show a decreased competitive binding to CRH2, whereas the L89S and F92S mutations have no effect on binding.