Fig. 8. Luciferase activity induced by a leptin mutant. Hek293T cells were transiently co-transfected with pMET7-mLRlo plasmid and the pXP2d2-rPAP1-luci reporter construct. The transfected cells were either stimulated with a dilution series of leptin mutant for 24 hours or left untreated. Luciferase measurements were performed in duplicate. The L86 mutant shows a shift of its EC50 value towards higher leptin concentrations.