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Fig. 2. Characterisation of CAP-D2 during mitosis. (A) CAP-D2 localises to a central axis in chromatids and shows accumulation at the centromeres from prometaphase until telophase. P-H3 was used as a mitotic marker. During late anaphase and telophase, when chromosomes begin to decondense, CAP-D2 still associates with chromosomes. CAP-D2 (red), P-H3 (green), and DNA (blue). (B) CAP-D2 accumulates at centromeres during metaphase. CID was used as a centromere marker. CAP-D2 (red), CID (green) and DNA (blue). (C) CAP-D2 is still strong in the daughter nuclei of a cell undergoing cytokinesis (arrow). CAP-D2 (red), {alpha}-tubulin (green) and DNA (blue).