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Fig. 2. AECs maintained in culture secrete a laminin-6-rich matrix. AECs at 4 days after isolation were processed for double-label indirect immunofluorescence using antibodies against the {alpha}3 laminin subunit (A,D) in combination with antibodies against either ß1 laminin (B) or {gamma}1 laminin (E). (G-I) Cells were processed with a mix of antibodies against {gamma}1 and ß1 laminin subunits, as indicated. (J-L) Cells were processed with a mix of antibodies against {alpha}3 and ß2 laminin subunit, as indicated. Specimens were viewed by confocal laser-scanning microscopy with the focal plane being as close as possible to the substrate-attached surface of the cells. Yellow color in the overlays in C,F,I marks colocalized protein. (L) The lack of yellow indicates the absence of colocalization of laminin subunits. Bar, 10 µm.