Fig. 3. Time course of LD formation (stained with BODIPY 493/503) in relation to ADRP and Rab18 in 3T3 cells loaded with oleic acid. Cells were cultured in medium containing 2% LPDS for 2 days, and then transferred to medium with 10% FBS with 200 µM oleic acid-BSA complex (OA/FBS). LDs, ADRP and Rab18 were not detected in the cells kept in LPDS. As early as 6 hours after transfer to OA/FBS medium, LDs and ADRP were observed as bright dots that increased in number and brightness thereafter. In contrast, Rab18 was barely visible as dots at 6 or 12 hours in OA/FBS medium, and was seen clearly only after 24 hours in OA/FBS medium.