Fig. 8. Golgi re-orientation is impaired in FAK knockdown cells. Rat-2 cells treated with either luciferase (A) or FAK (B) siRNA were subjected to a wound-healing assay for 4 hours, then stained for Golgi (red) and nuclei (blue), and analyzed for Golgi reorientation as described in Materials and Methods. The white line represents the location of the wound. Arrows in B indicate the disorganized Golgi in the FAK knockdown cells. (C) The percentages of control (white bars) and FAK knockdown (black bars) cells with Golgi orientated toward the wound were calculated at 2 and 4 hours following wounding of the monolayer. Error bars indicate standard deviation. *P<0.05 compared with the control bars.