Fig. 1. Downregulation of Alix expression in HeLa cells by siRNA. (A) HeLa cells were treated with control RNA (–) or with siRNA against Alix (+) for the incubation times indicated. The cells were lysed and analysed by SDS/PAGE and inmunoblotting with anti-Alix antibodies. Anti--tubulin antibodies were used to verify equal loadings. (B) Same experiment as in A, but the incubation time was 72 hours in the presence of siRNA, followed by 72 hours without siRNA. The amounts of Alix, Hrs, Tsg101 and -tubulin in cell lysates are shown.