Fig. 3. Alix depletion increases de colocalisation of early and late endosomal markers in the perinuclear region. HepII cells were treated with control RNA (A-D) or with siRNA against Alix (E-H) as in Fig. 1B. The cells were permeabilised before fixation and were stained with anti-EEA1 (A,E), anti-Hrs (B,F) and anti-CD63 (C,G). Scale bars: 5 µm.