Fig. 9. The F-actin structures that accumulate in Alix-depleted cells colocalise with clathrin. HeLa cells treated with control RNA (A,B) or with siRNA (C,D) against Alix (see Fig. 1B) were stained with rhodamine-phalloidin (A-C) and anti-clathrin (B-D). Yellow indicates colocalisation between actin and clathrin. Scale bars: 5 µm.