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Fig. 3. The Cabni1{Delta} mutant exhibited morphological defects during both yeast and hyphal growth. The yeast cells (A) of the wild-type (CaBNI1, strain WYL4) and the mutant (Cabni1{Delta}, strain WYL3) were grown in YPD at 30°C to exponential phase. The hyphal cells (B) were induced in YPD containing 10% serum at 37°C for 2 and 4 hours. (C) Cabni1{Delta} mutant exhibited random budding. The bud scars were visualized by Calcofluor staining. (D) Cells were grown on solid medium containing 0.05 mM ammonium sulphate for 3 days at 37°C. (E) Strains WYL23 (Cabnr1{Delta} CaBNI1) and WYL25 (Cabnr1{Delta} pMET3CaBNI1) were streaked onto two GMM plates, one containing 2.5 mM each of methionine (Met) and cysteine (Cys) and the other not. The plates were incubated at 30°C for 3 days. (F) WYL25 yeast cells were inoculated into liquid GMM supplemented with or without 2.5 mM methionine and cysteine and grown at 30°C for 5 hours (top). Then serum was added to each culture to a final concentration of 10% and the cells were incubated at 37°C for 3 hours (bottom). Bars, 5 µm.