Fig. 1. Effect of activated RhoB on endosome distribution and the actin cytoskeleton. Cells were microinjected with myc-RhoB^G14V (a-c), myc-RhoBwt (d-f) or myc-RhoA^G14V (g-i). After 4 hours incubation, cells were fixed and stained for myc, the endosomal protein EEA1 and F-actin. RhoB proteins largely co-localised with EEA1 on endosomes (a,b and d,e, arrowheads) while RhoA^G14V was cytosolic (g). EEA1-positive endosomes were located at the cell centre in control non-injected cells and in cells expressing RhoBwt (e) or RhoA^G14V (h). In cells expressing RhoB^G14V, EEA1-positive endosomes were peripheral and often appeared aligned on actin filaments (b,c, arrowheads). Bars: 10 µm.