Fig. 8. Activated RhoB effects on endosome distribution and actin coat assembly on endosomes are blocked by the dominant-negative Dia1-N1 mutant. GFP-mDia1-N1 (Dia1-N1) expression did not cause alteration of endosome distribution or the actin cytoskeleton (a-c). Dia1-N1 was recruited to endosomes by RhoB^G14V (compare cell co-expressing both proteins with cell expressing only Dia1-N1 (d,e)). Co-expression of Dia1-N1 with myc-RhoB^G14V blocked the effects of RhoB^G14V on the distribution of EEA1-positive endosomes, which localised to the juxtanuclear area as in non-injected cells (d-f). Inset in 8f shows a magnification of the merged picture of the injected cell in 8d-f showing white-blue vesicles positive for Dia1-N1 (green), RhoB (red) and EEA1 (blue). Dia1-N1 also blocked the assembly of an actin coat around RhoB^G14V-positive vesicles which now showed F-actin on a discrete site on their membranes (g-i). Inset in i shows vesicles labelled for RhoB (green) and F-actin (red). Bars: 10 µm.