Fig. 5. Ectopic furrows in klp67A mutants generate cytoplasts. Selected frames from a time-lapse sequence of a klp67A mutant during cytokinesis. At anaphase onset (0 minutes) the spindle was abnormally long and surrounded by a symmetrical cage of astral microtubules. These translocate during spindle elongation and coalesce at the right hand side and lower portion of the cell, where they initiate (12 minutes) the primary (arrows) and ectopic (16 minutes; arrowheads) cleavage furrows, respectively. Ingression of the two unilateral furrows leads to the formation of an anuclear cytoplast (26 minutes). Time is in minutes after anaphase onset. Bar, 10 µm.