Fig. 3. Influence of sexual maturation on the expression of gamone 1 during development. (A) The level of transcription of gamone 1 was examined in a progeny clone at each clonal age and in sexually mature type I cells (mature). The relative amounts of gamone 1 mRNA are indicated in the graph. For cells of clonal age of 10, 2.2 µg/lane was loaded, and for the others, 10 µg/lane. The extent of maturation at each clonal age is represented by the maturation index. These results suggested that the transcription of gamone 1 and maturation were strictly linked. (B) The biological activity of secreted mature gamone 1, represented in units.