Fig. 6. Influence of nutrient deprivation on the expression of the gamone 1 gene. (A) Total RNA was extracted from type I cells in the log phase [I (L)] and in the early stationary phase [I (1D)]. Northern hybridization showed that cells in the log phase did not transcribe the gamone 1 gene, but that early stationary phase cells started to transcribe it when they were moderately starved (1D). (B) The transcriptional level was compared for type I cells in the early stationary phase (1D) and cells starved in SMB^– for 5 days (5D). In both cells, the response to gamone 2 was also examined (G2–, untreated; G2+, treated with gamone 2). The transcription of gamone 1 was promoted in cells that had been starved longer (5D) and was accelerated by gamone 2 treatment. The graph shows the relative amounts of gamone 1 mRNA.