Fig. 10. uPAR is required for C5aR expression in mesangium upon LPS-induced renal inflammation in mice. (A) LPS-induced nephritis was induced in wild-type and uPAR^–/– mice as described in Fig. 9. 8 hours after challenge the mice were killed and 6 µm cryosections of kidney cortex were stained for C5aR protein expression using anti-C5aR primary antibody and Cy3-conjugated secondary antibodies. Representative microphotographs are shown (6 mice for each group). Bar, 50 µm. (B) Statistical analysis of C5aR expression in mesangium of wild-type and uPAR^–/– mice with LPS-induced nephritis was performed by evaluating the total number of positively stained glomeruli and the staining intensity (in arbitrary units) as described in the Materials and Methods. Results are expressed as mean ± s.e.m. (n=6 mice for each group). Significance between PBS controls and LPS-treated animals was determined by Student's t-test (*P<0.05; **P<0.01). +, arbitrary units (based on staining intensity using criteria described in Materials and Methods).