Fig. 2. uPAR mediates uPA-dependent upregulation of C5aR expression. The quiescent MCs were stimulated for 20 hours with 20 nM uPA in the presence or absence of 5 µg/ml anti-uPAR monoclonal antibody, and C5aR expression was investigated at the mRNA (A) and protein (B) levels using RT-PCR TaqMan analysis and western blotting, respectively. MCs incubated in medium without uPA and anti-uPAR antibody served as a control. The results in A are presented as mean ± s.e.m. for three separate and independent experiments. The data on the upper panel in B are representative of three separate and independent experiments. Quantification of the results of these experiments by densitometry presented as mean ± s.e.m. is shown in the lower panel. Significance between control unstimulated and stimulated cells was determined by Student's t-test (*P<0.05).