Fig. 3. uPA potentiates C5aR-related responses in MCs. (A) Quiescent MCs were incubated in 96-well microtiter plates with 20 nM uPA or 20 nM C5a alone or in combination with both stimuli in the presence or absence of 5 µg/ml anti-uPAR monoclonal antibody or irrelevant mouse IgG for 24 hours. The cells incubated in medium without stimuli served as a control. After 16 hours labeling with BrdU DNA synthesis was used as measure of the proliferation rate. Results are given as mean ± s.e.m. of four independent experiments performed in six parallel wells for each condition. (B) MCP-1 release was evaluated in supernatants from MC monolayers that were incubated as described in A for 48 hours. MCs incubated in medium without stimuli and producing 868±95 pg/ml MCP-1 served as a control. The data are given as mean ± s.e.m. of four separate and independent experiment performed in triplicate for each condition. Significance between control unstimulated and stimulated cells, as well as between the cells stimulated in the presence or not of 5 µg/ml anti-uPAR monoclonal antibody was determined by Student's t-test (*P<0.05; **P<0.01).