Fig. 8. uPAR utilizes gp130 adaptor protein to mediate C5aR expression in MCs. (A) Quiescent MCs were stimulated with 10 nM uPA for the indicated times or left unstimulated (control), and anti-gp130 (upper panel) or anti-uPAR (lower panel) antibody was used to coimmunoprecipitate gp130 and uPAR from the cell lysates. The immunoprecipitates were then analyzed with anti-uPAR (upper panel) or anti-gp130 antibody (lower panel). (B) RT-PCR analysis of C5aR mRNA was performed using the TaqMan method. RNA was isolated from quiescent MCs incubated for 6 hours with 20 nM uPA or in medium without uPA (control) in the presence or absence of 5 µg/ml of anti-gp130 antibody. Results are presented as mean ± s.e.m. of two independent experiments performed in duplicates for each condition.