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Fig. 5. Colocalization of SIAla21, SIAla22, SIAla31, SIAla32, SIAla33 and SIAla41 with ER, ERGIC and cis-Golgi in transfected COS-1 cells. The subcellular localization of fluorescent alanine scanning mutants of SI and intracellular markers was monitored as described for Fig. 3. Arrows indicate YFP fluorescence on the plasma membrane. n, nucleus. Bars, 25 µm.