Fig. 4. Localization of PAV-KLP in the female meiotic spindle. Oocytes were stained with anti-ß-tubulin (green) and anti-PAV-KLP (orange) antibodies. First meiosis: (A) anaphase, (B) late anaphase, (C) telophase: PAV-KLP accumulates at the middle of the spindle (arrow), at the presumptive midzone. Second meiosis: (D) prophase, (E) metaphase: the staining at the spindle midzone is no longer visible, but PAV-KLP localizes on a discrete ring-like structure at the spindle equator (arrows). (F) Anaphase: PAV-KLP concentrates at the focus of the central aster (arrow) and at the midzone of the twin spindles (arrowheads). Bar, 2 µm.