Fig. 7. PAV-KLP localization in cnn mutants. The merged images show PAV-KLP (orange) and MT (green) staining. (A) Anaphase I, (B) telophase of the first meiosis, (C) metaphase, (D) late anaphase of the second meiosis. PAV-KLP strongly accumulates at the central spindle during first meiosis (arrows), but the protein is barely detectable or absent within the focus of the faint central aster during the second meiosis (arrowheads); however, a discrete PAV-KLP labeling is found at the midzone of the twin meiotic spindles (small arrows). Bar, 2 µm.