Fig. 6. Expression of mycCRB3 in individual cells. MycCRB3-expressing MCF10A cells were grown on filters until 4 days post confluence, fixed, and co-stained for the myc epitope with the 4A6 anti-myc antibody (green) and for ZO-1 (red). Note that in contrast to the cells shown in other figures, these cells were permeabilized with 0.1% Triton X-100 instead of 1% SDS in order to visualize apical staining of mycCRB3. Triton X-100 permeabilization leads to slightly less sharp ZO-1 staining. A representative apical confocal section is shown. Arrowheads indicate tight junctions expressed in cells containing undetectable levels of mycCRB3.