Fig. 3. Latrunculin A affects the distribution and density of transmitted light structures. Chromaffin cell treated with latrunculin A (1 µM, 15 minutes) affects the integrity of the peripheral ring visualised with transmitted light scanning microscopy. Representative cell depicted before (A) and after (B) latrunculin A incubation. (C) Estimation of the effect by averaging the value of optical density in the cortical area for nine cells. (D,E) Time-course of the effect of latrunculin A on the internal organisation of transmitted light structures. Images were taken at 3-minute intervals (D) and mean image intensity (±s.e.m.) taken for each frame acquired. Bar, 10 µm (A,B); 1 µm (D).