Fig. 1. Strategy of PINCH1 gene disruption and embryos from PINCH1 heterozygous intercrosses. (A) Partial map of the wild-type PINCH1 allele, PINCH1neo, PINCH1-floxed allele (PINCH1fl), and the PINCH1-null allele after Cre-mediated recombination. Exons and loxP sequences are indicated as rectangles and triangles, respectively. The DNA fragment length obtained after Southern blotting and the internal (probe 1) and external (probe 2) probes are indicated. Restriction sites are: B1, BamHI; B2, BglII. (B) Southern blot of PINCH1neo tail DNA digestion with BamHI and hybridization with probe 2. The sizes of the wild-type (13 kb) and targeted (4 kb) bands are indicated. (C) PCR genotyping of E7.5 embryos derived from heterozygous intercrosses. (D) Whole-mount morphology of E7.5 wild-type and remnants of PINCH1-null embryos.