Fig. 2. Localisation of Bni1-YFP, Spa2-YFP and Bud6-GFP. (A) The fluorescence from Bni1-YFP merged with the DIC image of a germ tube. A single spot is observed just behind the tip (arrow). (B) Z-stack projection of hyphal cells expressing Spa2-YFP, which can be seen in all cells to form crescents or caps, with areas of higher intensity staining. (C) Localisation of Bud6-GFP, which predominantly forms a crescent, although in some cells a spot of higher fluorescence is observed (arrow). (D) Partial colocalisation of Spa2-YFP and Mlc1-CFP in hyphal cells. (E) Partial colocalisation of Spa2-YFP and FM4-64 in hyphal cells. Arrow indicates the tip that is enlarged in the model below. The model was constructed using the model-building module in the Softworx^TM suite. Spa2-YFP is shown as a green wireframe, FM4-64 is shown in solid red. The Spa2 cap covers the solid body of FM4-64. Bar, 5 µm (A); 1 µm (B,E); 2 µm (C,D).