Fig. 3. Localisation of YFP-Cdc42. (A-C) Cells expressing YFP-Cdc42 from the PCK1 promoter were grown in hyphal-inducing conditions and stained with FM4-64. The hyphal tip identified by the arrow in panel C is shown at greater magnification in panels D-F. Arrow in D indicates the subapical spot of YFP-Cdc42, which colocalises with FM4-64 resulting in the yellow spot in the merged image (F). Bar, 5 µm (A-C); 1 µm (D-F).